Abstract: For problems that existing uniqueness detection methods for personnel positioning easily did misjudgement and required on-site supervision, the paper proposed a design scheme of uniqueness detection system which combines iris recognition technology and RFID technology, and introduced system architecture, workflow and communication protocol design in details. The system identifies staff according to one to one correspondence of iris and radio frequency card, and automatically controls gate to achieve uniqueness detection, so as to achieve unattended attendance. The system is fast and accuracy in uniqueness detection, and achieves good effect in application in coal mine.