Abstract: In view of problems of low precision, low sampling rate and low data transmission rate existed in current data collection products based on USB interface in China, a data collection card with high performance and low noise was designed. The card uses high-speed differential amplifier AD8132 to make preamplifier; uses CPLD and SRAM to compose large capacity FIFO; uses USB2.0 bus technology to transmit data; uses independent clock to provide accurate synchronous clock signal for CPLD and AD converter to reduce clock phase noise; uses VC++ to design application program of upper computer to realize data analysis and processing. The test result showed that the card has advantages of low noise, rapid transmission rate and high precision.