Abstract: In view of problems that data acquisition card must be used to collect experimental data in use of virtual instrument and price of private data acquisition card is very high, the paper put forward a design scheme of data acquisition system based on LabVIEW and introduced the system's hardware configuration and software design. The system uses dual MCS51 control technology to achieve functions of on-line data acquisition or off-line mass data storage through USB interface, which can displace private data acquisition card.