Abstract: In this paper, the graphical programming language LABVIEW was introduced. The main functions and design ideas of the multi-function serial port debugging software based on LABVIEW were presented. Furthermore, module of data analysis of the serial port extended from the software was introduced. Through applying, it has proved that the software of serial port debugging and data analysis based on LABVIEW has several good characters such as powerful functions, easy for use, good transplant ability and good extensibility.