Abstract: According to impedance measurement method, a detection system of resonance frequency for magnetostrictive material based on AD5933 was designed. The hardware circuit and software design of the detection system were presented in details. The detection system uses impedance measurement module based on AD5933, microcontroller processing module based on MSP430F169, display module based on LCD 12864 and power supply module to realize data collection, processing, storage of resonance frequency of magnetostrictive material and impedance spectrum display. The test results show that the detection system has characteristics of low measurement error, strong anti-interference and high real-time performance, and average detection error is as low as 7 Hz.